Biotechnology

13.2

Biotechnology

Definition of Biotechnology

Biotechnology is a field which utilises technology or method to manipulate organisms for the production of biological products.

The Importance of Biotechnology

Biotechnology aims to improve the quality of livestock or crop products as well as to develop the use of microorganisms for a specific purpose.
Since biotechnology involves various disciplines, each activity that involves the use of organisms for a particular interest has been differentiated according to certain colors.
There are ten types of activities that have been identified, but green, white, yellow and blue biotechnology are the main activities in biotechnology.

Applications of Biotechnology in Life

Gene Therapy

Terapi gen digunakan untuk merawat atau mencegah suatu penyakit genetik.
Dalam terapi gen, gen yang normal disisipkan ke dalam gen pesakit untuk menggantikan gen yang abnormal.
Antara penyakit yang dapat dirawat menggunakan kaedah terapi gen ialah sistik fibrosis, distrofi otot dan beberapa jenis kanser.

DNA Profiling

DNA profiling is a forensic technique used to identify individuals based on their DNA.
This is said so because, DNA is unique to everyone, except identical twins.
The human DNA set is also different from the animal DNA.
By using DNA samples extracted from blood, semen or skin, an individual can be identified.
This DNA profiling process was invented by Sir Alec Jeffreys at the University of Leicester in 1985.
He was awarded the Copley medal for his accomplishments.
The Copley Medal was the oldest medal before the Nobel prize was introduced.
Application of DNA profilling:
- Identify criminal.
- Settle paternity and maternity dispute.
- Identify genetic disease.
- Determine suitability of organ donour and recipient.
Steps used in DNA profilling:
- Blood,semen or skin sample obtained from an investigation scene
- DNA is extracted from the sample.
- Restriction enzymes cut the DNA into different sizes of DNA fragments.
- The DNA fragments of different sizes are then separated by gel electrophoresis.
- DNA fragments of different sizes are transferred from the gel to a nylon membrane.
- Radioactive probes are added to the nylon membrane.X-ray film is then placed on top of the nylon membrane.
- X-ray film is processed to show the positions of DNA bands taht form the DNA profile.
DNA profiling can be used as a strong evidence in a criminal case if:
- The biological sample is obtained and processed correctly and consensusly.
- The forensic scientist tests the sample and analyses it meticulously in a transparent manner.
- The test results are interpreted precisely.
- The jury and judge obtain an accurate report.

Production of Insect Tolerant Plants

The use of thick cotton breeds that are tolerant to cotton pest caterpillars for example Bt cotton in cotton plantations has reduced the use of pesticides.
Bt plants are injected with a gene from Bacillus thuringiensis (Bt), a soil bacterium that can produce toxins.
This measure can save the cost of pesticides and increase cotton production.
Apart from cotton, other Bt crops are corn and eggplant.
The effect of Bt crops on pest is as shown below:
- Gene that produces Bt toxins from Bacillus thuringiensis is inserted into the plant’s genome.
- Pest feeds on the plant which produces Bt toxins.
- The toxins affect the digestive system of the pest and kills it.

Cleaning of Oil Spills

Bioremediation is a method of cleaning contaminants using bacteria
This method is effective for treating oil pollution due to oil spills in the ocean.
Most of the molecules in petroleum crude oil and purified petroleum products can be decomposed biologically using bacteria.
For example, Alcanivorax borkumensis, a type of bacteria that depends on oil for its source of energy is widely used to treat oil spills.

Importance of Biotechnology in Life

The field of biotechnology has grown rapidly in Malaysia.
This field makes a contribution in human life especially in developing countries and developed countries.
Biotechnology is often associated with agriculture, industry, nutrition, medicine and the like.
Rapid developments in the field of biology and technology have been able to replace the old methods used by ancient societies to more sophisticated methods, in addition to being able to diversify products that bring benefits to humans.
Biotechnology not only contributes to the increase in nutritional, agricultural and medical yields but also helps to develop a country's economy, especially in the industrial sector.

Contributions of biotechnology to life
Field	Contributions
Agriculture	Produce the best livestock and crop breeds.The tissue culture method and cloning technique have expedited plant reproduction. Produce paddy varities which give more yield and mature faster. Animal cloning enables mass reproduction and produces disease-resistant clones. Use of hormones expedite animal growth and maturity.
Medicine	Can modify the genetic contents of an individual. Reduce risk of mental disease. Prevent specific diseases such as cancer and inherited diseases. Human with dwarf genetics can have normal children. Assist mothers with diffuclty to conceive.
Forensic	Can identify criminal based on tissue or body fluid such as blood,semen,skin or hair follicle.
Environment	Microbes can expedite decay of waste products created by humans. Microbes can also degrade faeces completely. Reduce pollution to protect humans and the environenment.

Biotechnology

13.2

Biotechnology

Definition of Biotechnology

Biotechnology is a field which utilises technology or method to manipulate organisms for the production of biological products.

The Importance of Biotechnology

Biotechnology aims to improve the quality of livestock or crop products as well as to develop the use of microorganisms for a specific purpose.
Since biotechnology involves various disciplines, each activity that involves the use of organisms for a particular interest has been differentiated according to certain colors.
There are ten types of activities that have been identified, but green, white, yellow and blue biotechnology are the main activities in biotechnology.

Applications of Biotechnology in Life

Gene Therapy

Terapi gen digunakan untuk merawat atau mencegah suatu penyakit genetik.
Dalam terapi gen, gen yang normal disisipkan ke dalam gen pesakit untuk menggantikan gen yang abnormal.
Antara penyakit yang dapat dirawat menggunakan kaedah terapi gen ialah sistik fibrosis, distrofi otot dan beberapa jenis kanser.

DNA Profiling

DNA profiling is a forensic technique used to identify individuals based on their DNA.
This is said so because, DNA is unique to everyone, except identical twins.
The human DNA set is also different from the animal DNA.
By using DNA samples extracted from blood, semen or skin, an individual can be identified.
This DNA profiling process was invented by Sir Alec Jeffreys at the University of Leicester in 1985.
He was awarded the Copley medal for his accomplishments.
The Copley Medal was the oldest medal before the Nobel prize was introduced.
Application of DNA profilling:
- Identify criminal.
- Settle paternity and maternity dispute.
- Identify genetic disease.
- Determine suitability of organ donour and recipient.
Steps used in DNA profilling:
- Blood,semen or skin sample obtained from an investigation scene
- DNA is extracted from the sample.
- Restriction enzymes cut the DNA into different sizes of DNA fragments.
- The DNA fragments of different sizes are then separated by gel electrophoresis.
- DNA fragments of different sizes are transferred from the gel to a nylon membrane.
- Radioactive probes are added to the nylon membrane.X-ray film is then placed on top of the nylon membrane.
- X-ray film is processed to show the positions of DNA bands taht form the DNA profile.
DNA profiling can be used as a strong evidence in a criminal case if:
- The biological sample is obtained and processed correctly and consensusly.
- The forensic scientist tests the sample and analyses it meticulously in a transparent manner.
- The test results are interpreted precisely.
- The jury and judge obtain an accurate report.

Production of Insect Tolerant Plants

The use of thick cotton breeds that are tolerant to cotton pest caterpillars for example Bt cotton in cotton plantations has reduced the use of pesticides.
Bt plants are injected with a gene from Bacillus thuringiensis (Bt), a soil bacterium that can produce toxins.
This measure can save the cost of pesticides and increase cotton production.
Apart from cotton, other Bt crops are corn and eggplant.
The effect of Bt crops on pest is as shown below:
- Gene that produces Bt toxins from Bacillus thuringiensis is inserted into the plant’s genome.
- Pest feeds on the plant which produces Bt toxins.
- The toxins affect the digestive system of the pest and kills it.

Cleaning of Oil Spills

Bioremediation is a method of cleaning contaminants using bacteria
This method is effective for treating oil pollution due to oil spills in the ocean.
Most of the molecules in petroleum crude oil and purified petroleum products can be decomposed biologically using bacteria.
For example, Alcanivorax borkumensis, a type of bacteria that depends on oil for its source of energy is widely used to treat oil spills.

Importance of Biotechnology in Life

The field of biotechnology has grown rapidly in Malaysia.
This field makes a contribution in human life especially in developing countries and developed countries.
Biotechnology is often associated with agriculture, industry, nutrition, medicine and the like.
Rapid developments in the field of biology and technology have been able to replace the old methods used by ancient societies to more sophisticated methods, in addition to being able to diversify products that bring benefits to humans.
Biotechnology not only contributes to the increase in nutritional, agricultural and medical yields but also helps to develop a country's economy, especially in the industrial sector.

Contributions of biotechnology to life
Field	Contributions
Agriculture	Produce the best livestock and crop breeds.The tissue culture method and cloning technique have expedited plant reproduction. Produce paddy varities which give more yield and mature faster. Animal cloning enables mass reproduction and produces disease-resistant clones. Use of hormones expedite animal growth and maturity.
Medicine	Can modify the genetic contents of an individual. Reduce risk of mental disease. Prevent specific diseases such as cancer and inherited diseases. Human with dwarf genetics can have normal children. Assist mothers with diffuclty to conceive.
Forensic	Can identify criminal based on tissue or body fluid such as blood,semen,skin or hair follicle.
Environment	Microbes can expedite decay of waste products created by humans. Microbes can also degrade faeces completely. Reduce pollution to protect humans and the environenment.

Biotechnology

Biotechnology

Chapter : Genetic Technology

Topic : Biotechnology

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